Appearing in good spirits at the event alongside new husband Prince Harry, Meghan turned up at the event in a horse-drawn carriage smiling and waving to fans.
Once in the event, Meghan was stopped by Camilla, and the two appeared to exchange a series of tense words.
It's unclear what the two were talking about, but promptly after speaking, Meghan was instructed to follow behind Prince Charles and Camilla.
Later in the day, Meghan and husband Prince Harry were given the prestigious role of presenting jockey Frankie Dettori with his winning trophy following the St James' Palace Stakes race.
Meghan opted for a white shirt dress by Givenchy and accessorised with simple black and white accessories.
Harry and Meghan's attendance at Ascot marks a huge break away from tradition for the royals. William and Kate, the Duke and Duchess of Cambridge, did not attend the event together until 2016 – five years after they married.
It is believed they were invited every year, but politely declined until Catherine attended to celebrate the Queen’s 90th birthday in 2016.