The daughter of Prince Andrew and Sarah Ferguson accessorised with a snakeskin clutch and classic nude pointed stilettos.
Eugenie - who married her longtime love Jack Brooksbank in a fairytale royal wedding at St George's Chapel in Windsor last October - looked to be in high spirits as she chatted with her cousin Zara Tindall and cousin-in-law Autumn Phillips.
Eugenie's big sister Princess Beatrice, 30, was not seen on Ladies Day despite attending Royal Ascot earlier in the week.
But it wasn't all fun and games for Eugenie who was on hand to carry out a royal duty.
Fergie's daughter graciously presented a trophy to world renowned jockey Frankie Dettori who won the second race of the day, the Hampton Court Stakes, Town and Country Magazine reports.
It's been a busy week for Eugenie and her monarch grandmother who has attended each day of the historic racing festival.
On Thursday, Her Majesty the Queen looked far less than her 93 years in a glamorous dove grey outfit.
The Queen has ben accompanied by her children Prince Andrew, Duke of York and Princess Anne every day this week at Ascot Racecourse.