It's at this point where Karl, who's sitting next to wife Jasmine Stefanovic, appears visibly nervous as Gleeson goes in for the attack, but not before slamming Channel Nine reality TV show Married At First Sight.
"Congratulations to Married At First Sight by the way for their monster ratings. Give them a huge round of applause.
"Channel Nine were happy to cash in on your ratings by showing off your tacky weddings and fired Karl Stefanovic for having one!"
Karl took the joke on the chin, as Jasmine burst out laughing next to her husband.
While Gleeson was just poking fun at Karl, it seems the television personality agrees with the rest of Australia, declaring Karl should make his way back to Today.
"Karl, Today Show needs you. I was on the Today Show the other day to promote the Logies. The ratings are so low I felt like I was keeping a secret!"
Karl married his shoe designer wife, Jasmine, in an intimate Mexico ceremony back in December.