"This morning, I had her on my radio show, and I said: "What's the deal with your husband living in Melbourne trying to be an actor on Neighbours?", Kyle told The Project, "and she said: 'He's signed for a year.'"
Tim, who was once a chiropractor before appearing on the first ever season of The Bachelor Australia, permanently moved to Melbourne in March this year after landing a full-time role on Aussie soap, Neighbours.
"I said, 'hmm... I feel there's trouble there.'"
Kyle then mocked the Bachelor star's response, saying: "'No, no there's no trouble there.'"
"Watch that space. Something's going on!" Kyle added.
Trying to make up for his earlier blunt comments, Kyle admitted that Anna and Tim - who got married in a luxurious Italian ceremony last year - were a "super nice couple".
"They're a super nice couple, they're like what we all pretend we want to be, we just know it'll never be right."
That morning while on the Kyle and Jackie O show, Kyle spoke out about how he is 'worried' for the pair, telling his co-host Jackie O: "I think that Tim has abandoned her with the move down to Melbourne for Neighbours. She's still living in Sydney, and that worries me."
Tim responded to Kyle's comments, telling the Daily Mail Australia that he and Anna spend plenty of 'quality time' together.
"Anna and I knuckle down into work during the week and have plenty of quality time on the weekends," Tim said.
"We're actually surprised how well we've made the circumstances work.
"I was getting a little jealous of how much she was saying she loves Kyle but hopefully they just keep that romance in the courtroom."