JonBenét was only six years old when she was found murdered in her family home on December 26, 1996.
Investigators believed JonBenét was carried to the basement, silenced with duct tape, strangled with a garrote and smashed in the head.
The National Enquirer's exclusive report claims law enforcement sources strongly believe the killer's identity lies with JonBenét.
With modern technology and a worldwide DNA database at their disposal, investigators hope by repeating the process it will provide the forensic proof necessary to finally identify who was responsible.
The National Enquirer reports that John Ramsey originally opposed digging up JonBenét's remains despite multiple experts identifying tiny burn like marks on her body, potentially from a handheld stun gun.
In 2004, John described the mere though of exhuming his daughter as 'abhorrent.'
'We had buried our child. She was at peace. She was safe,' he said.
Now, John has reportedly admitted to the Enquirer that he 'regrets his decision' because the stun-gun evidence was 'overlooked during the autopsy' by the coroner.
He told the publication, 'At the time, I couldn't stand the thought of disturbing JonBenét so I said no, even though it would have perhaps shown the police they were wrong.
'Now, it would still be difficult for me, but if it was a compelling argument, I would consider it.'
The National Enquirer's latest expose follows the publication's recent report that widower Charlotte Hey, 85, confessed her husband Glenn Meyer, who lived across the street from the Ramsey home, was responsible for JonBenét's death.
Charlotte told the publication: 'When I asked him if he murdered her, he would just smile at me. He wouldn't deny it.'
In previous interviews, John Ramsey told the Enquirer that Meyer would turn up to his family home uninvited.
'Yes, Glenn Meyer was of interest at some point,' John said. 'I do recall him coming to our door the night of our family Christmas party, which was just before Christmas.'