Speaking to The Daily Telegraph, the radio host said: "We are living separately."
"I remain close to the family home and we made that decision to ensure our daughter's routine stays the same."
Jackie explained that Lee has continued doing the school drop-off's for their daughter as she wakes at 3:30am for her breakfast radio show.
Attempting to keep things as normal as possible for Kitty, the pair continue to have family dinners.
"We both get to enjoy time with her each day and our number one focus is ensuring there is as little disruption to her routine as possible," Jackie told the publication.
"We have family dinners together several nights a week, spend time together on weekends, we've recently gone away as a family and we plan on doing more of that in the future", she added.
Earlier in the week, speaking live on air, Jackie told listeners the decision "didn't happen overnight".
The couple tied the knot in 2003, and that they have been separated "for quite some time now".